Research Fellow in Biology

Humboldt University of Berlin Institute of Biology

Code number
DR / 195/20

Category (s)
Scientific staff

Number of digits
1

Place of use
Faculty of Life Sciences - Institute of Biology

application until
12/02/20

text

Job description:

Scientific services in a research project; The ultimate goal of the project is the light-driven generation of basic chemicals through the coupling of enzymes like formate dehydrogenase (FDH) or carbon monoxide dehydrogenase (CODH) to photosystem I (PSI) in solution to serve as a template for artificial applications. Therefore, the binding between the various modified PSI, FDH, CODH complexes, which are coupled by a linker is one of the most important tasks of the PhD in this project. The functional analysis of the direct coupling of PSI with FDH or CODH should take place in cooperation with Leimkühler (Universität Potsdam) and Dobbek (HU Berlin) groups. The PhD student is required for the optimizing the crystallization protocol of the native- and modified PSI for improvement of the structural data to at least 2.5 A resolution (P. Jordan et al, Nature, 2001). She/he will help with X-ray data collection at BESSY-II, Berlin, and functional interpretation of results, publication. She/he will develop a novel micro-seeding protocol to produce PSI and PSI-cyt c microcrystals for using the room temperature LCLS methods (Stanford, USA) in cooperation with the Berkeley group (USA). This implies that she/he participates in the LCLS-beamtimes (USA)

Requirements:

University degree in science (Master); knowledge and experience in working methods of molecular biology and biophysics, isolation of proteins via chromatography, characterization of proteins via spectroscopic methods (e.g. fluorescence spectroscopy), crystallization of proteins and crystallography; good English (spoken and written) skills are mandatory

Bewerbung an
Please send your application (including relevant certificates and two references), and quoting the reference number DR/195/20 to Humboldt-Universität zu Berlin, Faculty of Life Sciences, Institute of Biology, Frau Prof. Dr. Athina Zouni (Sitz: Philippstr. 13), Unter den Linden 6, 10099 Berlin or preferably to athina.zouni@hu-berlin.de.

The Humboldt-Universität zu Berlin is seeking to increase the proportion of women in research and teaching, and specifically encourages qualified female scholars to apply. Severely disabled applicants with equivalent qualifications will be given preferential consideration. People with an immigration background are specifically encouraged to apply. Since we will not return your documents, please submit copies in the application only.

Please visit our website www.hu-berlin.de/stellenangebote, which gives you access to the legally binding German version.

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